On-going research: In collaboration with Paul Monga, MD, Vice Chair Division of Experimental Pathology, my lab is using multi-photon-excitation (MPE) enabled intravital fluorescence microscopy of the liver in live mice to identify the molecular mechanism of blood-bile barrier failure during cholestasis and liver injury.
Primary research interest: The primary focus of my lab is to study the role of leukocytes and platelets in vaso-occlusion and organ injury associated with sickle cell disease. The current projects involve looking at the vaso-occlusive pathophysiology and acute chest syndrome in transgenic humanized sickle cell disease mice using intravital microscopy. The molecular pathways identified in mice are validated in humans by using blood samples from sickle patients in vascular mimetic in vitro microfluidic flow platforms and live cell microscopy.
Available techniques: Intravital microscopy in live mice, in vitro microfluidic platforms to test molecular targets in patient blood samples, live cell microscopy, and total internal reflection fluorescence microscopy.
Assistant Professor of Medicine – Division of Pulmonary, Allergy, and Critical Care Medicine
Principal Investigator – Pittsburgh Heart, Lung, and Blood, and Vascular Medicine Institute
Assistant Professor of Bioengineering, Swanson School of Engineering