Dr. Paul Monga, Professor of Pathology and Medicine, and colleagues publish a liver tissue clearing protocol in Star Protocols (Cell Press). PLRC member, Dr. Simon Watkins, also contributed to this protocol.
Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers.